Alternaria leaf and stem blight is a potential destructive foliar disease of sunflower in India and elsewhere causing significant yield losses during rainy season. Thirty-two interspecific lines derived from 11 Helianthus species were evaluated under natural epiphytotic conditions. Highly susceptible line L-101 was planted all along the borders and in between as an infector and spreader of the disease. The disease severity was assessed by taking 0-9 numerical rating system at three stages. The results indicated none of the wild derivatives showed resistant reaction. All the lines were highly susceptible to Alternaria leaf and stem blight.