Having established their efficiency and compatibility, the following organisms: Azotobacter chroococcurn (G A-2), Glomus fasciculatum ^nd Penicillium glaucum (HE-4) were selected to assess their combined effect on the growth and yield of sunflower in pot culture studies in greenhouse conditions, The results show that the values of growth parameters of sunflower like height, stem girth, number of leaves and leaf area were more responsive to all three organisms as compared with individual treatments. Early flowering is noticed in the treatment with Paricillium alone; leaf and stem dry weight were significantly higher than the control in all the treatments. All treatments e\cÊptAmtobacter alone resulted in significant increases in seed yield. The oil percentage was significantly over the control when test plants were inoculated with all three organisms together. However, all treatments had a positive effect on oil yield when compared with the control. Shoot nitrogen percentage was significantly increased over the control with all combinations of treatments. However, phosphorus percent did not vary in the treatments except in Glomus with Penicillium and all organisms together, where the phosphorus percent was higher than in the control. Rhizosphere study shows that the Amtobacler population was higher in the inoculated treatment. Phosphate-solubilising fungi were increased where P. melanogaster was inoculated with the other organisms. No large variations in the fungal population were noticed, whereas Glomus inoculation had stimulated the bacterial population. The percentages of mycorrhizal infection and spore production were lower in Glomus with Penicillium as compared with the other Glomus inoculation treatments.