Fertility restoration ability of the restorer was controlled by a single dominant gene. Bulked segregant analysis (BSA) was applied to identify molecular markers linked to a major restorer gene (Rf) using the F2 population of cms 234 A ×RHA 272. A total of 144 random oligo nucleotide primers were surveyed. The primer OPAM 061800 was found to produce putative markers, which differentiate parent and bulk from sterile parent and sterile bulk. The co-segregation analysis of the putative marker on the F2 population confirmed the association of OPAM 061800 produced by the primer OPAM 06 with the fertility restoration gene. This will help in transfering the fertility restorer gene to the inbreds lacking restoration genes.