By using the direct organogenesis method, applied for the first time at the Biotechnology Laboratory of DAI, General Toshevo, new sunflower forms were obtained through distant hybridization from the cross H. annuus L. (cv. Albena) × Helianthus tuberosus (Encheva et al., 1992). Three to eight hybrid plants were produced from a single embryo through the direct organogenesis method. This is a valuable method because it allows to obtain more than one plant from a hybrid embryo, something which is impossible with the com monly used “embryo rescue” technique. A considerable number of new sunflower lines were produced after self-pollination and individual selection. After characterization of the hybrid progenies according to seventeen morphological and biochemical indices, the conclusion can be drawn that lines R101 and R104 show 58.8% and 70.6%, respectively, intermediate phenotype in compar ison with the two parental forms. The positive transgressive forms were 29.4% and 23.5%, respectively, and the negative transgressive forms were 5.9% for both lines. Some of the R lines were directly included in heterosis breeding of sunflower.