Broomrape is the major issue in sunflower production in Serbia. Since its first occurrence, the species Orobanche cumana Wallr. had been responsible for considerable yield loss in some areas. In Serbia broomrape is present in the Vojvodina Province. Distribution and virulence groups of parasite are con stantly monitored. Only two distinct broomrape populations currently exist in this part of country. Race B is dominant in the south of the Vojvodina Province and race E in the north. During 2008, sets of 8 commercial hybrids with differ ent broomrape resistance genes were sown in regions with sunflower produc tion. Hybrids were sown on 34 different locations. Presence of broomrape was confirmed on 6 sites. Occurrence of parasitic plants was not detected on culti vars resistant to race E. Sunflower fields in regions where broomrape was not previously detected, were surveyed. The survey revealed occurrence of broom rapes in new regions. Infested areas are located in Bor County near the state border with Romania and Bulgaria.