Original article | Helia 2024, Vol. 47(80) 71-85
THE STUDY ON DETERMINATION OF FATTY ACID CONTENTS OF SOME WILD SUNFLOWER SPECIES (HELIANTHUS SPP.)
Yalçın Kaya, Semra Hasançebi, Emrah Akpınar, Müge Koç & Necmi Beşer
pp. 71 - 85 | DOI: https://doi.org/helia.2024.763.6 | Manu. Number: MANU-2411-24-0003
Published online: June 30, 2024 | Number of Views: 10 | Number of Download: 26
Abstract
Wild sunflower species have many sources of genes resistant to abiotic and biotic stresses, broomrape parasite as well as having higher quality traits. The transferring of these useful genes to the cultivated sunflower is so important issue to obtain a continuous resistance and then develop better quality and yielding cultivars in sunflower. The molecular methods in the plant breeding studies shortens the breeding cycles by providing an accurate and effective selection as well as saving time. The study was conducted in wild sunflower garden which set up via a project in previous years in Trakya University Edirne, Turkey. Fatty acid compositions of all wild species materials were determined in Trakya University laboratory for the first time in the world largely via Gas Chromatography (GC). The molecular analysis was performed in the lab to identify individuals with high oleic acid trait containing the homozygous oleic gene with 4 molecular markers (3 INDEL markers F4-R1, F4-R2 and F4-R3) and an SSR marker N1-3F) / (N2-1R HO). Based on GC analysis; no species with a high oleic rate (80% or more oleic acid) was found. However, among the examined wild species, three of them were found having mid oleic acid content (between 60 - 80% oleic acid). Among these species, Helianthus annuus species ranked first with the highest oleic acid content of 77.46%, followed by H. hirsutus with 69.71% and H. floridanus with 67.19%. On the other hand; Helianthus californicus, Helianthus exilis, Helianthus giganteus, Helianthus gracilentus, Helianthus grosseseratus, Helianthus laciniatus, Helianthus mollis, Helianthus neglectus, Helianthus petiolaris, Helianthus petiolaris subsp. petiolaris were determined as high oleic based on molecular 3 INDEL markers. However, these were not determined higher oleic acid content based on GC analysis. In conclusion; there was no genotype containing high oleic acid among the wild species based on GC analysis but high oleic species found in marker analysis; therefore, selectivity of the markers may not be accurate or new markers need to be used for the analysis of further researches.
Keywords: Wild sunflower, Fatty Acid, Oleic acid, Linoleic acid, Stearic acid, Palmitic acid, Molecular markers
| How to Cite this Article? |
|---|
|
APA 6th edition Harvard Chicago 16th edition |
| References |
|---|
|
Aşkın, B., Kaya, Y. 2020. Effect of deep frying process on the quality of the refined oleic / linoleic sunflower seed oil and olive oil. J. Food Science Technology. 57(12):4716–4725
|
