DNA marker for the <em>HaOr7</em> gene conferring resistance to the GRU race of broomrape (<em>Orobanche cumana</em> Wallr.) in sunflower.

Dmitrii Savichenko, Saida Guchetl & Elizaveta Loginova

doi:https://doi.org/10.29329/helia.2024.763.4

Original article | Helia 2024, Vol. 47(80) 36-51

DNA marker for the HaOr7 gene conferring resistance to the GRU race of broomrape (Orobanche cumana Wallr.) in sunflower.

Dmitrii Savichenko, Saida Guchetl & Elizaveta Loginova

pp. 36 - 51 | DOI: https://doi.org/10.29329/helia.2024.763.4 | Manu. Number: MANU-2410-14-0002

Published online: June 30, 2024 | Number of Views: 16 | Number of Download: 27

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Abstract

Sunflower broomrape (Orobanche cumana Wallr.) is an obligate parasite known for significantly reducing sunflower yield. The most widespread and virulent race of broomrape in the Russian Federation is the G race. The most ecologically and economically effective strategy for its control involves breeding genetically resistant sunflower varieties and hybrids. The use of DNA markers in the selection of resistant plants significantly enhances the efficiency of the breeding process. Two genetic markers, RORS1 for the HaOr7 gene, and the wild-type allele marker, SORS9, were designed to detect the presence or absence of a specific DNA fragment. A codominant marker system for multiplex PCR was developed from these markers. An analysis of 57 plants derived from the progeny of two heterozygous F1 hybrids revealed that the frequencies of phenotypic segregation aligned fully with the marker system, following the 3:1 model. All resistant plants exhibited amplification of the RORS1 marker, indicating the presence of the HaOr7 gene, while susceptible plants lacked this marker. Association analysis was conducted using the marker system, screening 90 susceptible and resistant sunflower lines and hybrids of different origins. Susceptible lines and hybrids were characterized by the amplification of the SORS9 marker only, whereas resistant lines exhibited amplification of the RORS1 marker only. Additionally, screening of resistant hybrids from eight different breeding companies cultivated in the Russian Federation revealed that all hybrids resistant to G and G+ races of broomrape carried the HaOr7 gene in a heterozygous state.

Keywords: DNA marker, broomrape resistance, marker-assisted selection

How to Cite this Article?

APA 6th edition
Savichenko, D., Guchetl, S. & Loginova, E. (2024). DNA marker for the HaOr7 gene conferring resistance to the GRU race of broomrape (Orobanche cumana Wallr.) in sunflower. . Helia, 47(80), 36-51. doi: 10.29329/helia.2024.763.4

Harvard
Savichenko, D., Guchetl, S. and Loginova, E. (2024). DNA marker for the HaOr7 gene conferring resistance to the GRU race of broomrape (Orobanche cumana Wallr.) in sunflower. . Helia, 47(80), pp. 36-51.

Chicago 16th edition
Savichenko, Dmitrii, Saida Guchetl and Elizaveta Loginova (2024). "DNA marker for the HaOr7 gene conferring resistance to the GRU race of broomrape (Orobanche cumana Wallr.) in sunflower. ". Helia 47 (80):36-51. doi:10.29329/helia.2024.763.4.

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