This study took into account the protein content in the kernel of ten lines derived from interspecific hybrids Helianthus annuus (line 2607) × Helianthus resinosus and Helianthus annuus (line 2607) × Helianthus salicifolius . The amino acid composition of storage protein was also studied. The protein in the new lines exceeded the protein in the parental forms with up to 10.6%. The essential amino acids lysine valine threonine and phenylalanine had higher amounts. With regard to sulfur-containing amino acids (cystine and methionine) the new lines had lower content than the line 2607. From the nonessential amino acids mainly asparagine and glycine exceeded the parental lines.