Over the past two decades there was observed a widespread of rust on sunflower in some regions of Russia. The identification of the racial structure of Puccinia helianthi populations in Russia has not been done since the early 80s of the last century. At that time races 100 and 300 were identified. In our recent study in addition to these races new biotypes: 700 710 722 772 were identified for the first time. The purpose of this study is to determine the racial identity of 160 P. helianthi isolates collected from 2018 to 2020 in the Rostov Saratov and Krasnodar regions. We used eight standard differentiating lines of sunflower: SM-90 SM 29 R-386 HАR-1 HАR-2 HAR-3 HAR-4 HAR-5. Sunflower variety VNIIMK 8883 was used as a differentiator susceptible to all races of the pathogen. In addition to the six races mentioned above we identified 11 more new races for the first time: 304 351 352 364 704 736 740 741 745 760 762. Race 700 prevailed among the isolates collected in the Rostov region in 2020; we also identified single specimens of races 760 762. Thus 17 races of P. helianthi were found on sunflower in three regions of the Russian Federation. Other races may be also present here so further research is required.