The presented results of the study on the effect of biopreparations and different fertilizer systems on the formation of sunflower yield and productivity determination of economic efficiency of sunflower cultivation under sharp fluctuations in climatic conditions through the use of biotechnology elements and fertilization. Growing sunflower without seed treatment with biopreparations under mineral fertilizer system provided an average yield of 2.93 t/ha with an additional 0.31 t/ha of sunflower seeds. The organo-mineral fertilizer system resulted in the highest increase in additional production – 0.50 t/ha and the increase in sunflower yield through fertilization system was the most significant with a minimum important difference (MID 05 ) of 0.11 t/ha. Seed treatment with biopreparation contributed to an increase in sunflower yield in all fertilizer systems: without fertilizer application only due to the action of biopreparation the yield increased to 2.82 t/ha; application of mineral fertilizer ensured a harvest of 3.09 t/ha of sunflower seeds; the organo-mineral fertilizer system had the highest yield indicator – 3.23 t/ha. The highest output of dry matter from the production was obtained through seed treatment with biopreparation and the use of organo-mineral fertilizer system which provided a yield per hectare of: grain units – 6.47 t fodder units – 3.56 t digestible protein – 1.23 t. However the most significant increase in sunflower productivity was observed in the organo-mineral fertilizer system without seed treatment with biopreparation. The highest indicators of economic efficiency of sunflower cultivation were observed under the conditions of seed treatment with biopreparation without fertilizer application which amounted to 18322 UAH/ha – net profit at a profitability of 91.5 %.